Haynes Lab:Notebook/Short Projects/2014/09/16: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span>
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==mm/dd/yy - First Last==
==09/16/14 - Jonah Thomas==
* Insert content here...
* Transformation - KAH60/pcVN into DH5α-turbo
 
 
----
 
'''Transformation'''
* HEK cells did not express mCherry after transfection
** Discarded all samples
* Therefore, we will try to obtain higher quality plasmid by transforming the stock into bacteria
* After transformation & growth, we will do a miniprep to get fresh plasmid DNA
** To check quality of DNA, we will do a restriction digest
 
Transformation samples
# KAH60/VNpc (from Harvard stocks) - 0.5 μL in 10 μL dH<sub>2</sub>O
# 10 μL dH<sub>2</sub>O
 
* Will check for colonies tomorrow morning




Latest revision as of 00:17, 27 September 2017

Haynes Lab - Short Projects Main project page
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09/16/14 - Jonah Thomas

  • Transformation - KAH60/pcVN into DH5α-turbo


Transformation

  • HEK cells did not express mCherry after transfection
    • Discarded all samples
  • Therefore, we will try to obtain higher quality plasmid by transforming the stock into bacteria
  • After transformation & growth, we will do a miniprep to get fresh plasmid DNA
    • To check quality of DNA, we will do a restriction digest

Transformation samples

  1. KAH60/VNpc (from Harvard stocks) - 0.5 μL in 10 μL dH2O
  2. 10 μL dH2O
  • Will check for colonies tomorrow morning



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