Haynes Lab:Notebook/Short Projects/2014/09/18: Difference between revisions
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'''Plasmid sequencing - planning''' | '''Plasmid sequencing - planning''' | ||
* Prep primers | |||
# P0001 (blue cap = 100 μM) | |||
# P0002 (blue cap = 100 μM) | |||
* Thaw stock primers (blue capped tubes) at room temp. | |||
* Get two sterile 1.5 mL tubes | |||
* Label each with the primer name and "10 μM" | |||
* Add 90 μL dH<sub>2</sub>O to each tube | |||
* Transfer 10 μL P0001 from blue caped tube into 90 μL H<sub>2</sub>O in new tube. Final volume = 100 μL, final conce. = 10 μM | |||
* Repeat last step for P0002. | |||
* Use DNASU: http://openwetware.org/wiki/Haynes:TechResources#Ordering | * Use DNASU: http://openwetware.org/wiki/Haynes:TechResources#Ordering | ||
Revision as of 11:53, 19 September 2014
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09/18/14 - Meli'sa Crawford
Plasmid sequencing - planning
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