Haynes Lab:Notebook/Short Projects/2014/09/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/21/14 - Meli'sa Crawford== | ||
* | * Restriction Digest of Plasmid DNA | ||
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'''Restriction Digest''' | |||
1. 60ml of 1x-TAE | |||
2. 6 grams Argarose (Low EEO) | |||
3. Restriction Enzymes: EcoRi and PSTL | |||
4. Buffer: FD Green Buffer | |||
5. 6.0μL of DNA cell stain | |||
6. 5.0μLof plasmid DNA | |||
7. 6.5μL of H<sub>2</sub>O | |||
* Chose the following plasmid DNA: BD003, BD004, BD005, BD006 | |||
* We know that the length of BD005 is 2422 bp | |||
*DPRE also known as D11.12 is 1740bp | |||
* We assume that BD006 must have the D11.12 construct. If this is so, the length of BD006 would be 4126bp | |||
Results: | |||
* BD005 and BD006 seemed to be the same length. | |||
* This could mean that BD006 could be the same plasmid DNA | |||
*May need to perform new restriction digest. | |||
* Buffer may have been too diluted. However, this couldn't effect the outcome of size. | |||
Latest revision as of 00:22, 27 September 2017
 Haynes Lab - Short Projects
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09/21/14 - Meli'sa Crawford
Restriction Digest 1. 60ml of 1x-TAE 2. 6 grams Argarose (Low EEO) 3. Restriction Enzymes: EcoRi and PSTL 4. Buffer: FD Green Buffer 5. 6.0μL of DNA cell stain 6. 5.0μLof plasmid DNA 7. 6.5μL of H2O
Results:
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