Haynes Lab:Notebook/Short Projects/2014/10/20

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10/20/14 - Jonah Thomas

  • Reviewed the results of the transfection mini-experiment (see below for results)
  • Plated new bacteria for KAH60/pcVn transduction



Transfection Mini-Experiment: Results

Note: Plate #1 was left as a control and Plate #2 was spun in a centrifuge (this was completed in hopes of increasing plasmid uptake)

  • Plate #1 and Plate #2 both showed RFP signal
    • The RFP signal from Plate #1 was stronger than the RFP signal from Plate #2
    • It is hypothesized that the cells on Plate #2 died because of their increased sensitivity due to spinning; in the future, p/s-free medium should be used to prevent this from happening

Microscope Images:

  • Plate #1, Well 1 - Transfected with 1160g KAH60/pcVn

RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment. RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment. RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment. RFP signal of mammalian cells transfected with KAH60/pcVn. This plate was the control group for the transfection mini-experiment.




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