Haynes Lab:Notebook/Short Projects/2015/03/01: Difference between revisions
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* Make 1 new T-75 at a 1:10 dilution | * Make 1 new T-75 at a 1:10 dilution | ||
* Passage number: 2 | * Passage number: 2 | ||
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* Make a batch of diluted cells for new 6-well plate: | * Make a batch of diluted cells for new 6-well plate: | ||
** Into a sterile 50 mL conical add... | ** Into a sterile 50 mL conical add... | ||
** (6 + 1 ''pipetting error'') x 0.25 mL stock cells = 1.75 mL stock cells | ** (6 wells + 1 ''pipetting error'') x 0.25 mL stock cells = 1.75 mL stock cells | ||
** (6 + 1 ''pipetting error'') x 3.75 mL fresh medium = 26.25 mL fresh medium | ** (6 wells + 1 ''pipetting error'') x 3.75 mL fresh medium = 26.25 mL fresh medium | ||
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'''Dox induction - KAH126''' | |||
* Induce cells in 6-well plate with dox to express PcTF (RFP) | |||
* Stock concentration of dox = 1.0 mg/mL | |||
* Desired final concentration of dox = '''1.0 μg/mL''' (1000x diluted) | |||
** Therefore '''add 1.0 μL of stock dox for every 1.0 mL medium in well''' | |||
* 4.0 mL medium in well x 1.0 μL stock dox = 4.0 μL stock dox to be added to each well | |||
Revision as of 16:59, 25 March 2015
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Karmella Haynes - 03/01/15
Passaging - KAH126
6-well plate - KAH126
Dox induction - KAH126
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