Haynes Lab:Notebook/Short Projects/2015/03/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab - Short Projects</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==03/03/15 - Alexander Ellingson== | ||
'''PCR-verify activation domain primers'''<br> | '''PCR-verify activation domain primers'''<br> | ||
Try different cDNA libraries for the CARM1 | Try different cDNA libraries for the CARM1 and MYB fragment to account for any mutations or deletions | ||
# U2OS C002, 1:1000 cDNA dilution; CARM1 = 1827 bp: CARM1 F1/ CARM1 | # U2OS C002, 1:1000 cDNA dilution; CARM1 = 1827 bp: CARM1 F1/ CARM1 R1; MYB = 159 bp: MYB F1/ MYB R1. | ||
# SKNSH C001, 1:1000 cDNA dilution; same. | # SKNSH C001, 1:1000 cDNA dilution; same. | ||
# K562 C001, 1:1000 cDNA dilution; same. | # K562 C001, 1:1000 cDNA dilution; same. | ||
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| bgcolor=#cfcfcf | Rxn2 | | bgcolor=#cfcfcf | Rxn2 | ||
| bgcolor=#cfcfcf | Rxn3 | | bgcolor=#cfcfcf | Rxn3 | ||
| rowspan="7" | Expected:<br>1. U2OS CARM1 = 1827 bp<br> | | rowspan="7" | Expected:<br>1. U2OS CARM1 = 1827 bp<br>7. U2OS MYB = 159 bp<br>2. SKNSH CARM1 = 1827 bp<br>8. SKNSH MYB = 159 bp<br>3. K562 CARM1 = 1827 bp<br>9. K562 MYB = 159 bp | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image: AQE3-4-15.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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* 4°C ∞ | * 4°C ∞ | ||
*Now running with the correct, 57°C annealing cycle. | |||
*Note: The device camera was not correctly functioning as of today; As a result, the picture attached was taken via camera phone. | |||
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Latest revision as of 00:48, 27 September 2017
Haynes Lab - Short Projects | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||
03/03/15 - Alexander EllingsonPCR-verify activation domain primers
Program: GOTAQ35cyc
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