Haynes Lab:Notebook/Short Projects/2015/05/29: Difference between revisions
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Transformation efficiency is pretty low. If plasmid prep/verification doesn't work, try longer transformation protocol using remaining ligation product. | Transformation efficiency is pretty low. If plasmid prep/verification doesn't work, try [http://openwetware.org/wiki/Haynes:TransformationPlasmids longer transformation protocol] using remaining ligation product. | ||
Revision as of 10:20, 29 May 2015
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Your Name - 29/05/15Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene Transformation results:
Transformation efficiency is pretty low. If plasmid prep/verification doesn't work, try longer transformation protocol using remaining ligation product.
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