12/17/13
- First trail run of OpenPCR & fluorimeter
Part 1: PCR
- Use the Open PCR machine to amplify segment from "CMV" plasmid DNA
- Program name = "First Trial 121713"
Reagent
|
1
|
2
|
3
|
4
|
5
|
2x PCR mix |
50 |
50 |
50 |
50 |
50
|
P0001 |
1 |
1 |
0 |
0 |
1
|
P0002 |
1 |
0 |
1 |
0 |
1
|
DNA template |
0.5 |
0.5 |
0.5 |
0.5 |
0
|
N.F. H2O |
47.5 |
48.5 |
48.5 |
49.5 |
48
|
Final vol |
100 |
100 |
100 |
100 |
100
|
- All components
- Forward primer + template DNA
- Reverse primer + template DNA
- Template DNA only
- Primers only
Part 2: Fluorimeter
- Fluorimeter had to be set up on one 96-hole PCR tube rack + two lids
- Zazu's camera phone was propped up in the black camera cradle with a blue lid from a 50-ml conical tube
- Diluted 100 uL PCR reactions by adding 500 uL molecular bio grade water (600 uL final volume, 6x dilution)
- On hydrophobic slide, applied 80 μL of diluted PCR sample, added 80 uL ready-to-use SYBR green dilution (from DNA day - provided by Dr. Garcia/ Rene Davis)
- Did this three times for sample #1 (all components) and sample #2 (forward primer + template DNA)
- Will continue with other samples after winter break
|