Haynes Lab:Notebook/SynBERC Scholars 2013/2014/02/14

From OpenWetWare

< Haynes Lab:Notebook | SynBERC Scholars 2013 | 2014 | 02(Difference between revisions)
Jump to: navigation, search
(Autocreate 2014/02/14 Entry for Haynes_Lab:Notebook/SynBERC_Scholars_2013)
Current revision (20:28, 14 February 2014) (view source)
(Entry title)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Procedure Digital PCR==
-
* Insert content here...
+
 +
'''2/14/14'''
 +
 +
*16 PCR Reactions were run today, following the procedure listed below.
 +
*TNF A forward and reverse primers were diluted from 100uM to 10uM. Initial stock DNA (Concentration = 2.3mg/mL) was diluted several times to obtain 4 different templates, listed below:
 +
*D0 = U20S 1:100 = 6.24ng/mL
 +
*D1 = 0.624ng/mL
 +
*D2 = 0.0624ng/mL = 6.24*10^(-2) ng/mL
 +
*D3 = 6.24pg/mL
 +
 +
 +
 +
*For each PCR reaction, the following volumes will be added:
 +
* 1uL of forward TNF A primer
 +
* 1uL of reverse TNF A primer
 +
* 1uL of diluted DNA (either U20S 1:100, D1, D2, or D3 for each sample)
 +
* 50uL of PCR master mix
 +
* 47 uL of dH20
 +
 +
*8 samples of each template will be put through the PCR  sequence (NOTE: The program to be run is named "First Trial 121713")
 +
*2 samples of plain water (instead of template) will also be tested as a negative control.
 +
 +
'''PCRs Run Today'''
 +
*8 D0 wells
 +
*8 D1 wells
 +
*Labeled and stored in freezer.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

Procedure Digital PCR

2/14/14

  • 16 PCR Reactions were run today, following the procedure listed below.
  • TNF A forward and reverse primers were diluted from 100uM to 10uM. Initial stock DNA (Concentration = 2.3mg/mL) was diluted several times to obtain 4 different templates, listed below:
  • D0 = U20S 1:100 = 6.24ng/mL
  • D1 = 0.624ng/mL
  • D2 = 0.0624ng/mL = 6.24*10^(-2) ng/mL
  • D3 = 6.24pg/mL


  • For each PCR reaction, the following volumes will be added:
  • 1uL of forward TNF A primer
  • 1uL of reverse TNF A primer
  • 1uL of diluted DNA (either U20S 1:100, D1, D2, or D3 for each sample)
  • 50uL of PCR master mix
  • 47 uL of dH20
  • 8 samples of each template will be put through the PCR sequence (NOTE: The program to be run is named "First Trial 121713")
  • 2 samples of plain water (instead of template) will also be tested as a negative control.

PCRs Run Today

  • 8 D0 wells
  • 8 D1 wells
  • Labeled and stored in freezer.


Personal tools