Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || <u> Master Mix </u> | | <u>Reagent</u> || <u>Volume</u> || <u> Master Mix </u> | ||
| rowspan="7" | <u>Expected:</u><br> | | rowspan="7" | <u>Expected:</u><br> KAH126 = 1880bps <br> MV2 = 4529bps<br> | ||
| rowspan="7" | [[Image:CA_Gel.jpeg|300px|E/P digests 8/29/12]]<br>15 μL/lane; 1% agarose | | rowspan="7" | [[Image:CA_Gel.jpeg|300px|E/P digests 8/29/12]]<br>15 μL/lane; 1% agarose | ||
| rowspan ="7" | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]<br>Gene Ruler | | rowspan ="7" | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]]<br>Gene Ruler | ||
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| dH<sub>2</sub>O || 8.5 || 25.5 | | dH<sub>2</sub>O || 8.5 || 25.5 | ||
|} | |} | ||
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#Put gel in waste box | #Put gel in waste box | ||
#SIZES! Compare to expected (MV2, HPCD...) > 2 bands | #SIZES! Compare to expected (MV2, HPCD...) > 2 bands | ||
<br> | |||
Analyzing the Gel | |||
*As a sanity check, prep 1 is more concentrated (as shown in the plate reading) and prep 2 is less bright because it is less concentrated | |||
*Our digest was done correctly. Checking with the ladder, the bands are lining up around the expected distances. (Check the 1500 and 4000 band lines on the ladder) | |||
*We see two bands because the plasmid was cut twice | |||
Latest revision as of 21:57, 26 September 2017
Project name | Main project page Previous entry Next entry | |||||||||||||||||||||
Digestion
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