Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/29

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Digestion

  • We usually use ECO-R1 or PST-1 to cut (refer to Dr. Hayes Biobrick cloning 12/16/09)
  • Part: BBa_J176122, Vector: MV2, Transfection Plasmid: KAH126


Reagent Volume Master Mix E/P digests 8/29/12
15 μL/lane; 1% agarose -->
DNA(plasmid) 3.0 μL -
10X buffer 1.5 4.5
EcoRI 1.0 3.0
PstI 1.0 3.0
dH2O 8.5 25.5
  15 μL --> 37°C/ ~15 min.


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