Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14
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5X diluted cells | 5X diluted cells | ||
| - | + | 1. 25mL diluted cells | |
*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down | ||
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
| - | + | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | |
Revision as of 12:33, 16 November 2012
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Splitting CellsSeed Culture
5X diluted cells 1. 25mL diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"
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