Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions
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5X diluted cells | 5X diluted cells | ||
1. 25mL diluted cells | 1. 25mL diluted cells | ||
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*5mL cells + 20mL medium in a 50mL conical vial | *5mL cells + 20mL medium in a 50mL conical vial | ||
*Pipette up and down | *Pipette up and down |
Revision as of 09:34, 16 November 2012
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Splitting CellsSeed Culture
5X diluted cells
1. 25mL diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"
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