Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14
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| - | == | + | ==Splitting Cells== |
| - | * | + | Seed Culture |
| + | # 12-well plate | ||
| + | # 75mL flask | ||
| + | |||
| + | 5X diluted cells | ||
| + | #25mL diluted cells | ||
| + | *5mL cells + 20mL medium in a 50mL conical vial | ||
| + | *Pipette up and down | ||
| + | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
| + | # 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
| + | |||
| + | |||
Revision as of 12:33, 16 November 2012
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Splitting CellsSeed Culture
5X diluted cells
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