Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions

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(Autocreate 2012/11/14 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
 
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==Entry title==
==Splitting Cells==
* Insert content here...
Seed Culture
# 12-well plate
# 75mL flask
 
5X diluted cells
#25mL diluted cells
*5mL cells + 20mL medium in a 50mL conical vial
*Pipette up and down
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
# 8mL medium + 2mL cells in 75mL flask labeled "PS4"
 
 




Revision as of 09:33, 16 November 2012

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Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells

  1. 25mL diluted cells
  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator
  1. 8mL medium + 2mL cells in 75mL flask labeled "PS4"



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