Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14
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< Haynes Lab:Notebook | Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2 | 2012 | 11(Difference between revisions)
(Autocreate 2012/11/14 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
Current revision (12:35, 16 November 2012) (view source) (→Splitting Cells) |
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| - | == | + | ==Splitting Cells== |
| - | + | '''Seed Culture''' | |
| + | # 12-well plate | ||
| + | # 75mL flask | ||
| + | '''5X diluted cells''' | ||
| + | <br/> | ||
| + | 1. 25mL diluted cells | ||
| + | *5mL cells + 20mL medium in a 50mL conical vial | ||
| + | *Pipette up and down | ||
| + | *Transfer 2mL of cells into each cell, store in 37 CO2 incubator | ||
| + | 2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | ||
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Splitting CellsSeed Culture
5X diluted cells
2. 8mL medium + 2mL cells in 75mL flask labeled "PS4" | |
