Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Splitting Cells==
==Splitting Cells==
Seed Culture
'''Seed Culture'''
# 12-well plate
# 12-well plate
# 75mL flask
# 75mL flask


5X diluted cells
'''5X diluted cells'''
1. 25mL diluted cells
<br/>
1. 25mL diluted cells  
*5mL cells + 20mL medium in a 50mL conical vial
*5mL cells + 20mL medium in a 50mL conical vial
*Pipette up and down
*Pipette up and down
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"
2.  8mL medium + 2mL cells in 75mL flask labeled "PS4"


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Latest revision as of 22:16, 26 September 2017

Project name Main project page
Previous entry      Next entry

Splitting Cells

Seed Culture

  1. 12-well plate
  2. 75mL flask

5X diluted cells
1. 25mL diluted cells

  • 5mL cells + 20mL medium in a 50mL conical vial
  • Pipette up and down
  • Transfer 2mL of cells into each cell, store in 37 CO2 incubator

2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"

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