Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/14 - Revision history

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:35:48 GMT

Splitting Cells

←Older revision		Revision as of 16:35, 16 November 2012
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	*Transfer 2mL of cells into each cell, store in 37 CO2 incubator		*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
	2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"		2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"
-
-
-

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:35:29 GMT

Splitting Cells

←Older revision		Revision as of 16:35, 16 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Splitting Cells==		==Splitting Cells==
-	Seed Culture	+	'''Seed Culture'''
	# 12-well plate		# 12-well plate
	# 75mL flask		# 75mL flask

-	5X diluted cells	+	'''5X diluted cells'''
-	~~1. 25mL diluted cells~~	+
	<br/>		<br/>
		+	1. 25mL diluted cells
	*5mL cells + 20mL medium in a 50mL conical vial		*5mL cells + 20mL medium in a 50mL conical vial
	*Pipette up and down		*Pipette up and down

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:34:50 GMT

Splitting Cells

←Older revision		Revision as of 16:34, 16 November 2012
Line 13:		Line 13:
	5X diluted cells		5X diluted cells
	1. 25mL diluted cells		1. 25mL diluted cells
-	</br>	+	<br/>
	*5mL cells + 20mL medium in a 50mL conical vial		*5mL cells + 20mL medium in a 50mL conical vial
	*Pipette up and down		*Pipette up and down

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:34:34 GMT

Splitting Cells

←Older revision		Revision as of 16:34, 16 November 2012
Line 12:		Line 12:

	5X diluted cells		5X diluted cells
-	1. 25mL diluted cells <br>	+	1. 25mL diluted cells
		+	</br>
	*5mL cells + 20mL medium in a 50mL conical vial		*5mL cells + 20mL medium in a 50mL conical vial
	*Pipette up and down		*Pipette up and down

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:34:14 GMT

Splitting Cells

←Older revision		Revision as of 16:34, 16 November 2012
Line 12:		Line 12:

	5X diluted cells		5X diluted cells
-	1. 25mL diluted cells	+	1. 25mL diluted cells <br>
	*5mL cells + 20mL medium in a 50mL conical vial		*5mL cells + 20mL medium in a 50mL conical vial
	*Pipette up and down		*Pipette up and down

Caroline Hom: /* Splitting Cells */

Caroline Hom — Fri, 16 Nov 2012 16:33:57 GMT

Splitting Cells

←Older revision		Revision as of 16:33, 16 November 2012
Line 12:		Line 12:

	5X diluted cells		5X diluted cells
-	#25mL diluted cells	+	1. 25mL diluted cells
	*5mL cells + 20mL medium in a 50mL conical vial		*5mL cells + 20mL medium in a 50mL conical vial
	*Pipette up and down		*Pipette up and down
	*Transfer 2mL of cells into each cell, store in 37 CO2 incubator		*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
-	# 8mL medium + 2mL cells in 75mL flask labeled "PS4"	+	2. 8mL medium + 2mL cells in 75mL flask labeled "PS4"

Caroline Hom: /* Entry title */

Caroline Hom — Fri, 16 Nov 2012 16:33:27 GMT

Entry title

←Older revision		Revision as of 16:33, 16 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Splitting Cells==
-	* ~~Insert content here...~~	+	Seed Culture
		+	# 12-well plate
		+	# 75mL flask
		+
		+	5X diluted cells
		+	#25mL diluted cells
		+	*5mL cells + 20mL medium in a 50mL conical vial
		+	*Pipette up and down
		+	*Transfer 2mL of cells into each cell, store in 37 CO2 incubator
		+	# 8mL medium + 2mL cells in 75mL flask labeled "PS4"
		+
		+

Caroline Hom: Autocreate 2012/11/14 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2

Caroline Hom — Fri, 16 Nov 2012 16:29:23 GMT

Autocreate 2012/11/14 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__
[[category:OWWLabNotebookV1]]