Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/11/30: Difference between revisions

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==Entry title==
==SK-N-SH Expansion==
* Insert content here...
* 2 new 75cm flasks for SK-N-SH


#1:5 passage (10mL fresh - 2mL cell, 8mL media)
#1:4 passage (10mL fresh - +8mL fresh to re-suspend cells)
'''Procedure'''
#Aspirate off media (titlt)
#Add 5mL of PBS to wash the cells
#Aspirate off PBS
#Add 2mL of trypsine (wash down walls) and let sit for 4-5 min
#Knock on container (a sufficient amount) to suspend cells
#Make the 2 75cm flasks as shown above, incubate


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Latest revision as of 22:19, 26 September 2017

Project name Main project page
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SK-N-SH Expansion

  • 2 new 75cm flasks for SK-N-SH
  1. 1:5 passage (10mL fresh - 2mL cell, 8mL media)
  2. 1:4 passage (10mL fresh - +8mL fresh to re-suspend cells)

Procedure

  1. Aspirate off media (titlt)
  2. Add 5mL of PBS to wash the cells
  3. Aspirate off PBS
  4. Add 2mL of trypsine (wash down walls) and let sit for 4-5 min
  5. Knock on container (a sufficient amount) to suspend cells
  6. Make the 2 75cm flasks as shown above, incubate
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