Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12

From OpenWetWare

Jump to: navigation, search
(Entry title)
Current revision (04:31, 20 December 2012) (view source)
(Primer Prep)
 
(One intermediate revision not shown.)
Line 8: Line 8:
==Primer Prep==
==Primer Prep==
* See excel document for number correlation
* See excel document for number correlation
-
#Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
+
#Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
-
#Heat @ 37C for ~5min, vortex
+
#Heat @ 37°C for ~5min, vortex
-
#In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)
+
#In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)
-
 
+
'''Tube Numbering (label number on tube top and concentration of solution on side'''
-
 
+
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]]
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37°C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)

Tube Numbering (label number on tube top and concentration of solution on side Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png

Personal tools