Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12: Difference between revisions

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#Heat @ 37C for ~5min, vortex
#Heat @ 37C for ~5min, vortex
#In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)
#In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)
 
'''Tube Numbering (label number on tube top and concentration of solution on side'''
 
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]]


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Revision as of 01:19, 20 December 2012

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Primer Prep

  • See excel document for number correlation
  1. Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer
  2. Heat @ 37C for ~5min, vortex
  3. In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution)

Tube Numbering (label number on tube top and concentration of solution on side

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