Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12
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< Haynes Lab:Notebook | Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2 | 2012 | 12(Difference between revisions)
(→Primer Prep) |
Current revision (04:31, 20 December 2012) (view source) (→Primer Prep) |
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==Primer Prep== | ==Primer Prep== | ||
* See excel document for number correlation | * See excel document for number correlation | ||
| - | #Add nm x 100 = | + | #Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer |
| - | #Heat @ | + | #Heat @ 37°C for ~5min, vortex |
| - | #In a fresh 1.5mL tube add | + | #In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution) |
'''Tube Numbering (label number on tube top and concentration of solution on side''' | '''Tube Numbering (label number on tube top and concentration of solution on side''' | ||
[[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] | [[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] | ||
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Primer Prep
Tube Numbering (label number on tube top and concentration of solution on side
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