# Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/12

(Difference between revisions)
 Revision as of 04:19, 20 December 2012 (view source) (→Primer Prep)← Previous diff Current revision (04:31, 20 December 2012) (view source) (→Primer Prep) Line 8: Line 8: ==Primer Prep== ==Primer Prep== * See excel document for number correlation * See excel document for number correlation - #Add nm x 100 = uL of dH2O (100uM stock in blue cap tube) to re-suspend primer + #Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer - #Heat @ 37C for ~5min, vortex + #Heat @ 37°C for ~5min, vortex - #In a fresh 1.5mL tube add 160uL of dH2O + 40uL of stock primer (20uL of 20uM working solution) + #In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution) '''Tube Numbering (label number on tube top and concentration of solution on side''' '''Tube Numbering (label number on tube top and concentration of solution on side''' [[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]] [[Image:Screen_Shot_2012-12-20_at_1.17.20_AM.png]]

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## Primer Prep

• See excel document for number correlation
1. Add nm x 100 = μL of dH2O (100μM stock in blue cap tube) to re-suspend primer
2. Heat @ 37°C for ~5min, vortex
3. In a fresh 1.5mL tube add 160μL of dH2O + 40μL of stock primer (20μL of 20μM working solution)

Tube Numbering (label number on tube top and concentration of solution on side