Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/18: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2012/12/18 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==Cells in Trizole== | ||
* | * Cells in trizole at 80°C | ||
'''K562''' | |||
#Expand cells to 10cm petri dishes for 10mL of cells | |||
#For the 6 plates, transfer 1mL cells to 2mL tubes 4 times (8mL total) | |||
#Spin tubes at room temperature @ 3000 RPM for 5 min | |||
#Dispose of supernatant | |||
#Add 500μL of trizol to each pellet | |||
#Store @ -80°C | |||
'''SK-N-SH''' | |||
#From the 6 well plate aspirate off the growth media | |||
#Add 500μL of trizol directly to the 6 well plate | |||
#Let sit for 5 min @ room temperature under the hood | |||
#Use 100m0mL pipet to scrape and pipet up and down to break up cells | |||
#Transfer 500μL into each tube | |||
Revision as of 11:38, 11 January 2013
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Cells in Trizole
K562
SK-N-SH
|