Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/19
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(Autocreate 2012/12/19 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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| - | == | + | ==SK-N-SH Expansion== |
| - | + | ||
| + | #1:5 passage PS5 (10mL fresh - 2mL cell, 8mL media) | ||
| + | #1:2 passage 6 well plate (Stock - 4mL cell, 4mL media; 1mL stock per well) | ||
| + | |||
| + | '''Procedure''' | ||
| + | #Aspirate off media (titlt) | ||
| + | #Add 5mL of PBS to wash the cells | ||
| + | #Aspirate off PBS | ||
| + | #Add 2mL of trypsine (wash down walls) and let sit for 4-5 min | ||
| + | #Knock on container (a sufficient amount) to suspend cells | ||
| + | #Prepare wells and flask as shown above | ||
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SK-N-SH Expansion
Procedure
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