Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/19: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2012/12/19 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==SK-N-SH Expansion== | ||
#1:5 passage PS5 (10mL fresh - 2mL cell, 8mL media) | |||
#1:2 passage 6 well plate (Stock - 4mL cell, 4mL media; 1mL stock per well) | |||
'''Procedure''' | |||
#Aspirate off media (titlt) | |||
#Add 5mL of PBS to wash the cells | |||
#Aspirate off PBS | |||
#Add 2mL of trypsine (wash down walls) and let sit for 4-5 min | |||
#Knock on container (a sufficient amount) to suspend cells | |||
#Prepare wells and flask as shown above | |||
Revision as of 01:30, 20 December 2012
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SK-N-SH Expansion
Procedure
|