Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/12/19

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SK-N-SH Expansion

  1. 1:5 passage PS5 (10mL fresh - 2mL cell, 8mL media)
  2. 1:2 passage 6 well plate (Stock - 4mL cell, 4mL media; 1mL stock per well)

Procedure

  1. Aspirate off media (titlt)
  2. Add 5mL of PBS to wash the cells
  3. Aspirate off PBS
  4. Add 2mL of trypsine (wash down walls) and let sit for 4-5 min
  5. Knock on container (a sufficient amount) to suspend cells
  6. Prepare wells and flask as shown above


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