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Project name
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RNeasy Mini Kit
- Add 100μL of chloroform to each tube
- Shake vigorously for 20 sec and let sit for 2 min
- Centrifuge in cold room for 15 min @ 12,000G
- Pipet off clear phase from organic phase into new tube (warning: do not get pink stuff in the tube!)
- Add 70% ethanol into tube (1:1 ratio)
- Transfer to collection tube (we have K562 1, K562 2, SK-N-SH 1, and SK-N-SH 2)
- Spin tubes at top speed for 30 sec
- Discard collected liquid and add 700μL of RW1 Buffer
- Spin tubes at top speed for 30 sec
- Put collection vial in a fresh collector tube and discard of the old one
- Add 500μL of RBE Buffer
- Spin tubes at top speed for 30 sec then discard of the liquid waste
- Repeat the above two steps again
- Discard of the waste and spin again
- Transfer liquid to a fresh tube and add 30mL of RNase-Free H20
- Let tubes sit for 1 min
- Spin tubes for 1 min
- Store at -80°C
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