Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/01/10: Difference between revisions
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'''Preparation''' | '''Preparation''' | ||
* Work with samples on ice | * Work with samples on ice | ||
* Ice RNase | * Ice: RNase H, SS III RT, and RNase out | ||
* Room temp: Oligo nT, RT Buffer, MgCl2, dNTP mix, water, DTT | * Room temp: Oligo nT, RT Buffer, MgCl2, dNTP mix, water, DTT | ||
'''Sample Reading''' | '''Sample Reading''' | ||
* Add | * Add 2μL of each sample plus blank to the plate reader | ||
# K562 Sample 1: 0.44 (260), 2.043 (260/280), 352.1 (ng/mL) | # K562 Sample 1: 0.44 (260), 2.043 (260/280), 352.1 (ng/mL) | ||
# K562 Sample 2: 0.385, 2.035, 308.3 | # K562 Sample 2: 0.385, 2.035, 308.3 |
Revision as of 18:52, 10 January 2013
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cDNAPreparation
Sample Reading
Part 1
Part 2
Part 3
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