Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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* Prepared rxn setup for plates
* Prepared rxn setup for plates


3x reaction:
3x reaction: <br/>
Probe master mix, (7.5 x3) 22.5 uL
Probe master mix, (7.5 x3) 22.5 uL <br/>
1:10 cDNA, (2.0 x3) 6.0 uL
1:10 cDNA, (2.0 x3) 6.0 uL <br/>
dH2O, (0.2 x3) 0.6 uL
dH2O, (0.2 x3) 0.6 uL <br/>
Total = (9.7 x3) 29.1
Total = (9.7 x3) 29.1
 
<br/>
<br/>
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
 
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<br/>
*Note: To dilute cDNA add 10uL of cDNA to 90uL of water
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Latest revision as of 22:28, 26 September 2017

Project name Main project page
Previous entry      Next entry

Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).

  • Note: To dilute cDNA add 10uL of cDNA to 90uL of water
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