Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Prepared rxn setup for plates | * Prepared rxn setup for plates | ||
3x reaction: | 3x reaction: <br/> | ||
Probe master mix, (7.5 x3) 22.5 uL | Probe master mix, (7.5 x3) 22.5 uL <br/> | ||
1:10 cDNA, (2.0 x3) 6.0 uL | 1:10 cDNA, (2.0 x3) 6.0 uL <br/> | ||
dH2O, (0.2 x3) 0.6 uL | dH2O, (0.2 x3) 0.6 uL <br/> | ||
Total = (9.7 x3) 29.1 | Total = (9.7 x3) 29.1 | ||
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Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | ||
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*Note: To dilute cDNA add 10uL of cDNA to 90uL of water | |||
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Latest revision as of 22:28, 26 September 2017
Project name | Main project page Previous entry Next entry |
Assay Design
3x reaction:
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