Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20

Assay Design

• Designed 6 plates for each cell line
• Prepared rxn setup for plates

3x reaction:
Probe master mix, (7.5 x3) 22.5 uL
1:10 cDNA, (2.0 x3) 6.0 uL
dH2O, (0.2 x3) 0.6 uL
Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).

• Note: To dilute cDNA add 10uL of cDNA to 90uL of water