Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20
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(Autocreate 2013/02/20 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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| - | == | + | ==Assay Design== |
| - | * | + | * Designed 6 plates for each cell line |
| + | * Prepared rxn setup for plates | ||
| + | |||
| + | 3x reaction: | ||
| + | Probe master mix, (7.5 x3) 22.5 uL | ||
| + | 1:10 cDNA, (2.0 x3) 6.0 uL | ||
| + | dH2O, (0.2 x3) 0.6 uL | ||
| + | Total = (9.7 x3) 29.1 | ||
| + | |||
| + | Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | ||
Revision as of 14:27, 22 February 2013
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Assay Design
3x reaction: Probe master mix, (7.5 x3) 22.5 uL 1:10 cDNA, (2.0 x3) 6.0 uL dH2O, (0.2 x3) 0.6 uL Total = (9.7 x3) 29.1 Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
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