Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20: Difference between revisions
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Caroline Hom (talk | contribs) (Autocreate 2013/02/20 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
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== | ==Assay Design== | ||
* | * Designed 6 plates for each cell line | ||
* Prepared rxn setup for plates | |||
3x reaction: | |||
Probe master mix, (7.5 x3) 22.5 uL | |||
1:10 cDNA, (2.0 x3) 6.0 uL | |||
dH2O, (0.2 x3) 0.6 uL | |||
Total = (9.7 x3) 29.1 | |||
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | |||
Revision as of 11:27, 22 February 2013
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Assay Design
3x reaction: Probe master mix, (7.5 x3) 22.5 uL 1:10 cDNA, (2.0 x3) 6.0 uL dH2O, (0.2 x3) 0.6 uL Total = (9.7 x3) 29.1 Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).
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