Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20
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< Haynes Lab:Notebook | Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2 | 2013 | 02(Difference between revisions)
(Autocreate 2013/02/20 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2) |
Current revision (14:32, 22 February 2013) (view source) (→Assay Design) |
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| - | == | + | ==Assay Design== |
| - | * | + | * Designed 6 plates for each cell line |
| - | + | * Prepared rxn setup for plates | |
| + | 3x reaction: <br/> | ||
| + | Probe master mix, (7.5 x3) 22.5 uL <br/> | ||
| + | 1:10 cDNA, (2.0 x3) 6.0 uL <br/> | ||
| + | dH2O, (0.2 x3) 0.6 uL <br/> | ||
| + | Total = (9.7 x3) 29.1 | ||
| + | <br/> | ||
| + | <br/> | ||
| + | Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before). | ||
| + | <br/> | ||
| + | <br/> | ||
| + | *Note: To dilute cDNA add 10uL of cDNA to 90uL of water | ||
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Assay Design
3x reaction:
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