Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20

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(Autocreate 2013/02/20 Entry for Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Gene2)
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==Entry title==
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==Assay Design==
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* Insert content here...
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* Designed 6 plates for each cell line
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* Prepared rxn setup for plates
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3x reaction:
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Probe master mix, (7.5 x3) 22.5 uL
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1:10 cDNA, (2.0 x3) 6.0 uL
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dH2O, (0.2 x3) 0.6 uL
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Total = (9.7 x3) 29.1
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Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).

Revision as of 13:27, 22 February 2013

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Assay Design

  • Designed 6 plates for each cell line
  • Prepared rxn setup for plates

3x reaction: Probe master mix, (7.5 x3) 22.5 uL 1:10 cDNA, (2.0 x3) 6.0 uL dH2O, (0.2 x3) 0.6 uL Total = (9.7 x3) 29.1

Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).


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