Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2013/02/20
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3x reaction: Probe master mix, (7.5 x3) 22.5 uL 1:10 cDNA, (2.0 x3) 6.0 uL dH2O, (0.2 x3) 0.6 uL Total = (9.7 x3) 29.1
Add: (0.3 x3) 0.9 uL UPL probe and (5.0x3) 15.0 uL primer pair mix in appropriate wells. Aliquot 15 uL into the triplicate wells (same as before).