Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Genes/2012/06/17: Difference between revisions

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==Gene Coordinated==
==Gene Coordinates/Additional Cell Lines==
* These will be the gene coordinates (+/-250bps of the promoter region) to be used every time a calibration for a cell line is needed
* These will be the gene coordinates (+/-250bps of the promoter region) to be used every time a calibration for a cell line is needed
* Note: These coordinates are based of of the RefSeq genes
* Note: These coordinates are based off of the RefSeq genes
[[Image:Screen_Shot_2012-06-17_at_8.47.49_PM.png]]
[[Image:Screen_Shot_2012-06-17_at_8.47.49_PM.png]]
*Behzad's U2OS cell line, which is cancerous, is having problems picking up any signal for commonly known silenced genes.
*I ran a calibration on other cancerous cell lines, and I seem to be having the same problem. Refer to paper: http://cancerres.aacrjournals.org/content/67/11/5097.full
[[Image:Screen_Shot_2012-06-17_at_8.53.25_PM.png]]





Revision as of 20:55, 17 June 2012

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Gene Coordinates/Additional Cell Lines

  • These will be the gene coordinates (+/-250bps of the promoter region) to be used every time a calibration for a cell line is needed
  • Note: These coordinates are based off of the RefSeq genes

  • Behzad's U2OS cell line, which is cancerous, is having problems picking up any signal for commonly known silenced genes.
  • I ran a calibration on other cancerous cell lines, and I seem to be having the same problem. Refer to paper: http://cancerres.aacrjournals.org/content/67/11/5097.full