Haynes Lab:Notebook/TB Biosensor/2012/05/24: Difference between revisions

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==Entry title==
==Entry title==
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The following parts are Amp resistant and from the bacteria, Pantoea ananatis. The numbers correspond to the labeling used in the lab:


1. K118014;    RBS + crtE                                                                2012 kit plate 2 well 18H   http://partsregistry.org/Part:BBa_K118014)
2. K274100; RBS + crtE + RBS + crtI + RBS + crtB                  2012 kit plate 3 well 6H   http://partsregistry.org/Part:BBa_K274100
3. K274200; RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 2012 kit plate 3 well 6L   http://partsregistry.org/Part:BBa_K274200
4. K118002; crtB                                                                         2012 kit plate 2 well 16F   http://partsregistry.org/Part:BBa_K118002
5. K118006; RBS + crtB                                                                 2012 kit plate 2 well 16N   http://partsregistry.org/Part:BBa_K118006
6. I742161; RBS + crtI + RBS + crtB                                         2012 kit plate 2 well 20C   http://partsregistry.org/Part:BBa_I742161
7. K118003; crtI                                                                         2012 kit plate 2 well 16H   http://partsregistry.org/Part:BBa_K118003
8. K118005; RBS + crtI                                                                 2012 kit plate 2 well 16L   http://partsregistry.org/Part:BBa_K118005
I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 36deg (maybe 30? check warm room temp).


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Revision as of 10:27, 25 May 2012

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Entry title

The following parts are Amp resistant and from the bacteria, Pantoea ananatis. The numbers correspond to the labeling used in the lab:

1. K118014; RBS + crtE 2012 kit plate 2 well 18H http://partsregistry.org/Part:BBa_K118014) 2. K274100; RBS + crtE + RBS + crtI + RBS + crtB 2012 kit plate 3 well 6H http://partsregistry.org/Part:BBa_K274100 3. K274200; RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 2012 kit plate 3 well 6L http://partsregistry.org/Part:BBa_K274200 4. K118002; crtB 2012 kit plate 2 well 16F http://partsregistry.org/Part:BBa_K118002 5. K118006; RBS + crtB 2012 kit plate 2 well 16N http://partsregistry.org/Part:BBa_K118006 6. I742161; RBS + crtI + RBS + crtB 2012 kit plate 2 well 20C http://partsregistry.org/Part:BBa_I742161 7. K118003; crtI 2012 kit plate 2 well 16H http://partsregistry.org/Part:BBa_K118003 8. K118005; RBS + crtI 2012 kit plate 2 well 16L http://partsregistry.org/Part:BBa_K118005

I pipetted 10ul of water into each well in the kit plates and transfered the DNA to 1.5ml tubes. I added 30ul of thawed DH5alpha competent cells to each tube, gently mixed, and left the tubes on ice for 5 minutes. I then pipetted the cells onto LB+amp plates and left overnight at 36deg (maybe 30? check warm room temp).


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