Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions
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Plasmids from 12.05.29: | Plasmids from 12.05.29: | ||
{| class="wikitable" border="0" cellspacing="3" <!-- | {| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Lane</u> || <u>Part Number</u> || <u>Gene(s) | | <u>Lane</u> || <u>Part Number</u> || <u>Gene(s)</u> | ||
|- | |- | ||
1 || K118014 || RBS + crtE | |-1 || K118014 || RBS + crtE | ||
|- | |- | ||
2 || K274100 || RBS + crtE + RBS + crtI + RBS + crtB | |-2 || K274100 || RBS + crtE + RBS + crtI + RBS + crtB | ||
|- | |- | ||
3 || K274200 || RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY | |-3 || K274200 || RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY | ||
|- | |- | ||
4 || K118002 || crtB | |-4 || K118002 || crtB | ||
|- | |- | ||
5 || K118006 || RBS + crtB | |-5 || K118006 || RBS + crtB | ||
|- | |- | ||
6 || I742161 || RBS + crtI + RBS + crtB | |-6 || I742161 || RBS + crtI + RBS + crtB | ||
|- | |- | ||
7 || K118003 || crtI | |-7 || K118003 || crtI | ||
|- | |- | ||
8 || K118005 || RBS + crtI | |-8 || K118005 || RBS + crtI | ||
|} | |} | ||
Revision as of 17:01, 8 June 2012
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Entry titlePCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift). Plasmids from 12.05.29:
PCR reaction
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