Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 15: Line 15:
| <u>Lane</u> || <u>Part Number</u> || <u>Gene(s)</u>  
| <u>Lane</u> || <u>Part Number</u> || <u>Gene(s)</u>  
|-
|-
|-1 || K118014 || RBS + crtE
|-1 || K118014 || RBS + crtE
|-
|-
|-2 || K274100 || RBS + crtE + RBS + crtI + RBS + crtB
|-2 || K274100 || RBS + crtE + RBS + crtI + RBS + crtB

Revision as of 17:04, 8 June 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

PCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift).

Plasmids from 12.05.29:

Lane Part Number Gene(s)


Gene Plasmid Primer F Primer R


PCR reaction

Reagent Volume
DNA (plasmid) 1.0
10 μM primers 1.0
2x GoTaq 25.0
dH2O 23.0