Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2012/06/08 Entry for Haynes_Lab:Notebook/TB_Biosensor) |
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==Entry title== | ==Entry title== | ||
PCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift). | |||
Plasmids from 12.05.29: | |||
Lane Part Number Gene(s) | |||
1 K118014 RBS + crtE | |||
2 K274100 RBS + crtE + RBS + crtI + RBS + crtB | |||
3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY | |||
4 K118002 crtB | |||
5 K118006 RBS + crtB | |||
6 I742161 RBS + crtI + RBS + crtB | |||
7 K118003 crtI | |||
8 K118005 RBS + crtI | |||
Gene Plasmid Primer F Primer R | |||
crtE 1 | |||
crtE 2 | |||
crtE 3 | |||
crtB 2 | |||
crtB 3 | |||
crtB 4 | |||
crtB 5 | |||
crtI 2 | |||
crtI 3 | |||
crtI 7 | |||
crtI 8 | |||
PCR reaction | |||
Revision as of 16:45, 8 June 2012
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Entry titlePCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift). Plasmids from 12.05.29: Lane Part Number Gene(s) 1 K118014 RBS + crtE 2 K274100 RBS + crtE + RBS + crtI + RBS + crtB 3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 4 K118002 crtB 5 K118006 RBS + crtB 6 I742161 RBS + crtI + RBS + crtB 7 K118003 crtI 8 K118005 RBS + crtI Gene Plasmid Primer F Primer R crtE 1 crtE 2 crtE 3 crtB 2 crtB 3 crtB 4 crtB 5 crtI 2 crtI 3 crtI 7 crtI 8
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