Haynes Lab:Notebook/TB Biosensor/2012/06/08: Difference between revisions

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1 K118014  RBS + crtE
1 K118014  RBS + crtE
2 K274100 RBS + crtE + RBS + crtI + RBS + crtB
2 K274100 RBS + crtE + RBS + crtI + RBS + crtB
3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY
3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY
4 K118002 crtB
4 K118002 crtB
5 K118006 RBS + crtB
5 K118006 RBS + crtB
6 I742161 RBS + crtI + RBS + crtB
6 I742161 RBS + crtI + RBS + crtB
7 K118003 crtI
7 K118003 crtI
8 K118005 RBS + crtI
8 K118005 RBS + crtI


Gene  Plasmid  Primer F  Primer R
Gene  Plasmid  Primer F  Primer R
crtE       1
crtE       1
crtE        2
crtE        2
crtE        3
crtE        3
crtB       2
crtB       2
crtB        3
crtB        3
crtB        4
crtB        4
crtB        5
crtB        5
crtI       2
crtI       2
crtI        3
crtI        3
crtI        7
crtI        7
crtI        8
crtI        8



Revision as of 16:46, 8 June 2012

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Entry title

PCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift).

Plasmids from 12.05.29:

Lane Part Number Gene(s)

1 K118014 RBS + crtE

2 K274100 RBS + crtE + RBS + crtI + RBS + crtB

3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY

4 K118002 crtB

5 K118006 RBS + crtB

6 I742161 RBS + crtI + RBS + crtB

7 K118003 crtI

8 K118005 RBS + crtI

Gene Plasmid Primer F Primer R

crtE 1

crtE 2

crtE 3

crtB 2

crtB 3

crtB 4

crtB 5

crtI 2

crtI 3

crtI 7

crtI 8


PCR reaction