Haynes Lab:Notebook/TB Biosensor/2012/06/08

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PCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift).

Plasmids from 12.05.29:

Lane Part Number Gene(s)

1 K118014 RBS + crtE 2 K274100 RBS + crtE + RBS + crtI + RBS + crtB 3 K274200 RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 4 K118002 crtB 5 K118006 RBS + crtB 6 I742161 RBS + crtI + RBS + crtB 7 K118003 crtI 8 K118005 RBS + crtI

Gene Plasmid Primer F Primer R crtE 1 crtE 2 crtE 3 crtB 2 crtB 3 crtB 4 crtB 5 crtI 2 crtI 3 crtI 7 crtI 8


PCR reaction




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