Haynes Lab:Notebook/TB Biosensor/2012/06/08

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PCR'd the plasmids cut on 12.05.29 to PCR out the individual genes with the correct overhangs (Silver Standard RFC[23] compatible with no frame shift).

Plasmids from 12.05.29:

1 || K118014 || RBS + crtE 2 || K274100 || RBS + crtE + RBS + crtI + RBS + crtB 3 || K274200 || RBS + crtE + RBS + crtB + RBS + crtI +RBS + crtY 4 || K118002 || crtB 5 || K118006 || RBS + crtB 6 || I742161 || RBS + crtI + RBS + crtB 7 || K118003 || crtI 8 || K118005 || RBS + crtI
Lane Part Number Gene(s) F


Gene Plasmid Primer F Primer R
crtE 1 P001 P002
crtE 2 P001 P002
crtE 3 P001 P002
crtB 2 P003 P004
crtB 3 P003 P004
crtB 4 P003 P004
crtB 5 P003 P004
crtI 2 P005 P006
crtI 3 P005 P006
crtI 7 P005 P006
crtI 8 P005 P006


PCR reaction

Reagent Volume
DNA (plasmid) 1.0
10 μM primers 1.0
2x GoTaq 25.0
dH2O 23.0



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