Haynes Lab:Notebook/TB Biosensor/2012/09/13: Difference between revisions

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{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->
{| class="wikitable" border="0" cellspacing="3" <!-- 12.05.29 plasmids. table -->
|-valign="top"
|-valign="top"
| <u>Template</u> || <u>Forward Primer</u> || <u>Reverse Primer</u>  
| <u>Final Construct </u> || <u>Template</u> || <u>Forward Primer</u> || <u>Reverse Primer</u>  
|-
|-
| crtE || P001 || P009
| crtE - PLFlex - X || crtE || P001 || P010 ||
|-
|-
| crtE ||   || P002 ||
| X- PLFlex - crtE || crtE || P015  || P002 ||
|-
|-
| crtI || P005 ||
| crtI - PLFlex - X ||  crtI  || P005 || P012 ||
|-  
|-  
| crtI || || P006 ||
| X- PLFlex - crtI || crtI || P017 || P006 ||
|-
|-
| crtB  ||  P007 ||
| crtB - PLFlex - X || crtB  ||  P007 || P014 ||
|-
|-
| crtB  ||   || P008 ||
| X- PLFlex - crtB || crtB  || P019 || P008 ||
|-
|-
| PLFlex ||  
| crtE - PLFlex - crtB || PLFlex || P009 || P020 ||
|-
|-
| PLFlex ||  
| crtE - PLFlex - crtI ||  PLFlex || P009 || P018 ||
|-
|-
| PLFlex ||  
| crtI - PLFlex - crtE || PLFlex || P011 || P016 ||
|-
|-
| PLFlex ||  
| crtI - PLFlex - crtB || PLFlex || P011 || P020 ||
|-
|-
| PLFlex ||  
| crtB - PLFlex - crtE || PLFlex || P013 || P016 ||
|-
|-
| PLFlex ||  
| crtB - PLFlex - crtI || PLFlex || P013 || P018 ||
|}
|}


Revision as of 16:03, 13 September 2012

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Making crt Fusion Proteins by Gibson Assembly

  • Ernesto PCR'd the overhangs onto the crt genes for Gibson assembly.


Final Construct Template Forward Primer Reverse Primer
crtE - PLFlex - X crtE P001 P010
X- PLFlex - crtE crtE P015 P002
crtI - PLFlex - X crtI P005 P012
X- PLFlex - crtI crtI P017 P006
crtB - PLFlex - X crtB P007 P014
X- PLFlex - crtB crtB P019 P008
crtE - PLFlex - crtB PLFlex P009 P020
crtE - PLFlex - crtI PLFlex P009 P018
crtI - PLFlex - crtE PLFlex P011 P016
crtI - PLFlex - crtB PLFlex P011 P020
crtB - PLFlex - crtE PLFlex P013 P016
crtB - PLFlex - crtI PLFlex P013 P018


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