Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/01/29: Difference between revisions
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== | ==Mara Peterson - 01/29/15== | ||
'''Session 2''' | |||
Growing Liquid Cultures of Transformed Cells | |||
Result for Plate 1: over 60 growth colonies; | |||
Result for Plate 2 (Negative Control): no growth | |||
List of samples for liquid cultures: | |||
(1) Culture 1 = Colony 1 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance | |||
(2) Culture 2 = Colony 2 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance | |||
(1) and (2) are each in 14-mL culture tubes an incubator to be left overnight, with an Amp agar plate with a streak of (1) and a streak of (2). | |||
Revision as of 10:47, 25 March 2015
 Haynes Lab Cloning Bootcamp
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Mara Peterson - 01/29/15Session 2 Growing Liquid Cultures of Transformed Cells Result for Plate 1: over 60 growth colonies; Result for Plate 2 (Negative Control): no growth List of samples for liquid cultures: (1) Culture 1 = Colony 1 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance (2) Culture 2 = Colony 2 from Plate 1, DH5a-Turbo cells, KAH126/MV2 plasmid, Amp resistance (1) and (2) are each in 14-mL culture tubes an incubator to be left overnight, with an Amp agar plate with a streak of (1) and a streak of (2).
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