Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/07/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab Cloning Bootcamp</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes Lab Cloning Bootcamp</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 12: Line 12:
Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.
Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.


Sample 1 Nanodrop results:


.02        .02


.008      .01
{| {{table}}
 
|- bgcolor=#cfcfcf
2.453    1.977
| Plasmid || OD260 || OD260/280 || ng/μL
 
|-
20.031  20.325
| 1. Plasmid 1 || .02 || 2.453 || 20.325
 
|-
 
| 2. Plasmid 2 || .187 || 1.911 || 187.22
Sample 2 Nanodrop results:
|}
 
.187     .182
 
.098      .097
 
1.911   1.882
 
187.22 182.42


=Plasmid Validation Template page=
=Plasmid Validation Template page=
Line 55: Line 45:
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = 746, 2155 <br>2. Plasmid 2 = 746, 2155<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = 746, 2155 <br>2. Plasmid 2 = 746, 2155<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:Bdbootcamp.jpeg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA(plasmid) || 3.0 μL
| DNA(plasmid) || 3.0 μL

Latest revision as of 01:04, 27 September 2017

Haynes Lab Cloning Bootcamp Main project page
Previous entry      Next entry

Brady Dennison - 07/28/15

Sample 1: Liquid culture grew, but showed no signs of the mCherry gene. Plate had growth and showed the mCherry gene.

Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.


Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 .02 2.453 20.325
2. Plasmid 2 .187 1.911 187.22

Plasmid Validation Template page

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 746, 2155
2. Plasmid 2 = 746, 2155
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:





Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook:Shared/Cloning_Bootcamp/2015/07/29&oldid=1017064"