Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/07/29: Difference between revisions

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Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.
Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.


Sample 1 Nanodrop results:


.02        .02
.008      .01
2.453    1.977
20.031  20.325
Sample 2 Nanodrop results:
.187      .182
.098      .097
1.911    1.882
187.22  182.42


{| {{table}}  
{| {{table}}  

Revision as of 12:29, 29 July 2015

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Brady Dennison - 07/28/15

Sample 1: Liquid culture grew, but showed no signs of the mCherry gene. Plate had growth and showed the mCherry gene.

Sample 2: Liquid culture grew, showed signs of mCherry gene. Plate also had growth and showed the mCherry gene.


Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 .02 2.453 20.325
2. Plasmid 2 .187 1.911 187.22

Plasmid Validation Template page

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 746, 2155
2. Plasmid 2 = 746, 2155
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:





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