HighPoint/CannonLab:Fluorescence Spectrometry: Difference between revisions
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# Turn ON the spectrometer | # Turn ON the spectrometer | ||
#* look for the <span style="color:#FF0000">red Lamp Monitor LED</span> on front of the instrument to turn on | #* look for the <span style="color:#FF0000">red Lamp Monitor LED</span> on front of the instrument to turn on | ||
#* wait for the LED on the right side of the instrument to change from <span style="color: | #* wait for the LED on the right side of the instrument to change from <span style="color:darkorange">ORANGE</span> to <span style="color:green">GREEN</span> | ||
# Turn on the computer | # Turn on the computer | ||
#* user "lab", password "lab" | #* user "lab", password "lab" | ||
Latest revision as of 09:37, 6 February 2012
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Research=#,Protein Membrane=Protein Membrane Interactions, Osmolytes=Osmolyte Effects
Protocols=#, Vesicles=Lipid Vesicle Preparation, Solutions=Buffer and Osmolyte Solutions, Fluorescence=Fluorescence Spectrometry, UV-Vis=UV-Vis Spectrometry, Pipetting=Pipetting Exercise
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Using the Fluorometer
Warnings
- Open the sample chamber to close the shutters and protect the detector if the signal exceeds the maximum (~9).
- Turn off the lamp before shutting down the instrument.
- Be careful with the cuvettes. You will break one if you work enough, but please develop careful habits.
Using the SLM Aminco-Bowman Luminescence Spectrometer
Startup
- Make sure the computer is OFF
- Turn ON the "Lamp Enable" switch on the left side of the spectrometer
- Turn ON the spectrometer
- look for the red Lamp Monitor LED on front of the instrument to turn on
- wait for the LED on the right side of the instrument to change from ORANGE to GREEN
- Turn on the computer
- user "lab", password "lab"
- Open AB2 program
- ignore the error message
- wait for "Initializing Instrument" to finish
- follow the remainder of instructions beginning on p. 4-1 of the manual
Shutdown
- 20 Minutes before turning off the spectrometer turn OFF the "Lamp Enable" switch
- This is crucial to extend the life of the expensive arc lamp
- Turn off the computer at any time
- Clean the cuvettes and return them to the rack behind the computer
- These are the other expensive and breakable item
- They are placed behind the computer when not in use to minimize chances of accidental damage
