Hoatlin: Fundamentals: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 18: | Line 18: | ||
==Questions and Answers== | ==Questions and Answers== | ||
;Question | |||
*I am a bit confused about the DNA polymerases that you mention on slide 56 of your lecture. Are the leading and lagging strand synthesied by different polymerases? Secondly, you mention polymerase eta and its role in synthesis of the leading strand. I thought eta was only involved in the bypass mechanism? Thanks for your clarification and help. | |||
;Answer | |||
*I think I see the confusion. I believe it is partly confusion over greek letters used to name the pols. Pol eta (looks like an italics small n) is a translesion pol---so you are correct. Slide 56 mentions pol '''epsilon''' (Greek letter looks like like an italics e) which is the replicative pol that has recently been shown to primarily replicate the leading strand in eukaryotes. The lagging strand is synthesized primarily by pol delta. This division of labor at the replication fork in eukaryotes is a recent discovery, so many texts will have it backwards. Note that in eukaryotes there are two replicative pols: polymerase delta and epsilon, whereas in prokayotes there is only one main replicative polymerase, Pol III. Primase is required in both systems. | |||
;Question | ;Question | ||
*I am still having trouble understanding PARPi's mechanism. Can you explain to me the normal interactions and mutated interactions of PARP and BRCA along with PARPi's function? | *I am still having trouble understanding PARPi's mechanism. Can you explain to me the normal interactions and mutated interactions of PARP and BRCA along with PARPi's function? |
Revision as of 16:08, 29 January 2011
Home Projects Team Papers Contact Us Protocols Collaborations News Reagent Requests secret back door Hoatlin Lab Twitter
Links for Maureen Hoatlin's CSF 2011 ClassBonus Materials
Really.
Questions and Answers
That's how PARP1 inhibitors are relatively selective in killing the HR deficient tumor cell but not the wild-type cell (which is competent for HR)
Other Stuff |