Hop DNA Isolation

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Revision as of 16:32, 5 March 2007

Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
Assume 90% of mass is water weight.
Add

Buffer: 100mL: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/mL of CTAB and 20muL/mL 2-mercaptoethanol.

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-	Buffer: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM	+	Buffer: 100mL: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/mL of CTAB and 20muL/mL 2-mercaptoethanol.