How To Make A MEGA-plate: Difference between revisions
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==Overview== | ==Overview== | ||
This page provides step-by-step instructions for the MEGA-Plate experiment for evolving and tracking microbial evolution on antibiotic gradients {ref}. | This page provides step-by-step instructions for the MEGA-Plate experiment for evolving and tracking microbial evolution on antibiotic gradients {ref}. | ||
The MEGA-plate was developed by | The MEGA-plate was developed by | ||
Michael Baym, Tami Liemberman, Eric Kelsic, Remy Chait, Rotem Gross, Idan Yelin, Roy Kishomy | Michael Baym, Tami Liemberman, Eric Kelsic, Remy Chait, Rotem Gross, Idan Yelin, Roy Kishomy |
Revision as of 07:20, 12 January 2017
Overview
This page provides step-by-step instructions for the MEGA-Plate experiment for evolving and tracking microbial evolution on antibiotic gradients {ref}.
The MEGA-plate was developed by Michael Baym, Tami Liemberman, Eric Kelsic, Remy Chait, Rotem Gross, Idan Yelin, Roy Kishomy Kishony Lab at Harvard Medical School and Technion - Israel Institute of Technology
Materials
For a plate of 62cm on 82cm:
- Polycarbonate sheet 62cm X 82cm X 0.6cm (compeny,ID)
- Plexiglas sheet 30cm X 60cm X 0.3cm (compeny,ID)
- Dichloromethane (compeny,ID)
- Syringe (compeny,ID)
- Needle (compeny,ID)
- 9 X 2L Erlenmeyer flask (compeny,ID)
- Agar (http://www.bd.com/ds/productCenter/214010.asp#)
- LB Broth (http://www.bd.com/ds/productCenter/240220.asp)
Procedure
- Plan the design of the plate.
- Use laser cutter to cut the plexiglas sheet to your design:
- 8 pieces of 40cm X 1.27cm
- 4 pieces of 40cm X 2cm
- 2 pieces of 60cm X 2cm
- Glue the pieces like the plan design.
- Check for leaking.
- Use blith
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
- Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
- Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164
Contact
This page was developed by Michael Baym and Rotem Gross from the Kishony lab. For questions, please contact: Michael Baym (mbaym@gmail.com) Rotem Gross () Roy Kishony (rkishony@technion.ac.il)
or instead, discuss this protocol.